Immunochemical Studies on β 1 - Bungarotoxin

....................................................................................................................................................... 79 INTRODUCTION ................................................................................................................................................. 80 NUMBER OF ANTIGENIC DETERMINANTS ON β1-Bgt ................................................................................. 80 β1-Bgt-NEUTRALIZING EPITOPE MAPPING Determination of the binding site of seven β1-Bgt-neutralizing mAbs ...................................................... 81 Neutralizing epitope mapping of β1-Bgt with synthetic peptide and proteolytic method .......................... 82 Characterization of the neutralizing epitopes of β1-Bgt ............................................................................ 83 PREPARATION AND CHARACTERIZATION OF β1-Bgt BISPECIFIC mAb .................................................... 85 Production of hybrid hybridoma and preparation of bispecific mAb ......................................................... 85 Characterization of anti-β1-Bgt bispecific mAb ......................................................................................... 85 Mechanism of avidity enhancement by bispecific mAb ............................................................................ 87 NEUTRALIZING EPITOPE MAPPING AS A BASIS FOR β1-Bgt PEPTIDE VACCINE DESIGN ..................... 87 Protective capacity of peptide-immunized mice ........................................................................................ 87 Reduction of mortality by passively transferred anti-peptide sera or peptide-specific antibodies ........... 88 CONCLUSIONS .................................................................................................................................................. 88 ACKNOWLEDGMENTS .................................................................................................................................... 89 REFERENCES ................................................................................................................................................... 89 CHINESE ABSTRACT ....................................................................................................................................... 90